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Molecular cha- racteristics of apple mosaic virus isolates on hops (Humulus lupulus L.) in Belarus
Abstract
The article presents the results of application of the PCR method for the diagnosis of Belarusian Apple mosaic virus isolates (ApMV) in hop. It was established that the PCR method with the use of a pair of primers to the gene of viral coat protein (PAPMCP-5/PAPMCP-3) can successfully be used to detect ApMV in hop in Belarus. As a result of the molecular genetic analysis, sections of genomes of three Belarusian ApMV isolates in hop were sequenced for the first time, the nucleotide sequence of which was placed in the international database (EMBL/GenBank) with identification numbers: LT546544, LT546545 and LT546546. Phylogenetic analysis of nucleotide sequences of isolates of ApMV in hop showed that the grouping of the isolates did not depend on their geographical origin. The analyzed virus isolates formed three subgroups, and the Belarusian isolates were in two of them.
About the Authors
O. A. Gasenko
Институт плодоводства
Belarus
Belarus
N. N. Volosevich
Университет Масарика
Czech Republic
Czech Republic
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Review
For citations:
Gasenko O.A., Volosevich N.N. Molecular cha- racteristics of apple mosaic virus isolates on hops (Humulus lupulus L.) in Belarus. Сrop Farming and Plant Growing. 2017;(6):31-35. (In Russ.)
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