Molecular-genetic characteristics of Rhizoctonia spp., causing edge eye spot of grain crops in Belarus
Abstract
This paper describes a method for isolating of total DNA from Rhizoctonia spp. mycelium and PCR (polymerase chain reaction) techniques for the identification of R. cerealis and R. solani species using selected species-specific molecular markers. Here are the examples of PCR – detection of these fungi presented in the collection of isolates collected in Belarus by the Institute of Plant Protection.
About the Authors
Е. А. Voluevich
Институт генетики и цитологии
Russian Federation
Russian Federation
V. I. Luksha
Институт генетики и цитологии
Russian Federation
Russian Federation
A. G. Zhukovsky
Институт защиты растений
Russian Federation
Russian Federation
V. G. Leshkevich
Институт защиты растений
Russian Federation
Russian Federation
References
1. Lemanczyk, G. Use of SCAR-PCR in diagnostics of stem base pathogens of the Rhizoctonia and Oculimacula genus /G. Lemanczyk // Acta agrobotanica. – 2011. – Vol. 64, № 4. – P. 197–206.
2. Nicholson, P. Development and use of a PCR assay to detect Rhizoctonia cerealis, the cause of sharp eyespot in wheat / P. Nicholson, D.W. Parry // Plant Pathol. – 1996. – Vol. 45. – P. 872–883.
Review
For citations:
Voluevich Е.А., Luksha V.I., Zhukovsky A.G., Leshkevich V.G. Molecular-genetic characteristics of Rhizoctonia spp., causing edge eye spot of grain crops in Belarus. Сrop Farming and Plant Growing. 2015;(4):50-53. (In Russ.)
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