Induction of protective answer genes in potato leaves at bacterial infection and glyphosate treatment
Abstract
The protective proteins genes expressive levels are determined in potato leaves at bacterial infection and glyphosate treatment by real time PCR method. It is shown that transgenic plants treatment by glyphosate induces the expression in protective proteins genes leaves. At glyphosate treated plants contamination by phytopathogenic bacteria the protective answer genes induction levels are increased approximately two times. It is supposed that transgenic plants treatment by glyphosate facilitates their resistance increase to pathogens.
About the Authors
Ch. PasalariBelarus
O. М. Tretiakova
Belarus
А. N. Еvtushenkov
Belarus
References
1. Генетические трансформанты картофеля как модель изучения гормональной и углеводной регуляции клубнеобразования / Н. П. Аксенова [и др.] // Физиология растений. – 2000. – 47, 3: C. 420–430.
2. The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins / L. C. Van Loon [еt al.] // Physiol. Mol. Plant Pathol. 1999. – V. 55. – P. 85–97.
3. Пастухова, Н. Л. Детоксикация тяжелых металлов у растений / Н. Л. Пастухова // Available: http://repo.donnu.ru:8080/jspui/bitstream/123456789/3542/1/218-226.pdf [Accessed Okt. 05, 2010].
4. Малиновский, В. И. Механизмы устойчивости растений к вирусам / В. И. Малиновский. – Владивосток: Дальнаука, 2010. – C. 324.
5. Роль экстраклеточных рибонуклеаз в физиологических процессах высших растений / С. С. Сангаев [и др.] // Вестник ВОГиС. – 2010. – Т. 14., № 2. – С. 232–242.
6. Роль нуклеаз в физиологических процессах высших растений / Е. А. Трифонова [и др.] // Усп. соврем. биологии. – 2000. – Т. 120. – С. 395–405.
7. Основные свойства и особенности эволюции антифризных белков / Л. Л. Бильданова [и др.] // Вавиловский журнал генетики и селекции. – 2012. – Т. 16, № 1. – С. 250–270.
8. Экспрессия PR-генов при бактериальной инфекции / О. М. Третьякова [и др.] // Труды БГУ. – 2011. – Т. 6, № 1. – С. 163–167.
9. Activation of hsr203, a plant gene expressed during incompatible plant-pathogen interactions, is correlated with programmed cell death / D. Pontier [еt al.] // Mol. Plant-Microbe Interact. – 1998. – Vol. 11, № 6 – P. 544–554.
10. Discovery and directed evolution of a glyphosate tolerance gene / A. Linda [еt al.] // Science. – 2004. – Vol. 304. – P. 1151–1154.
11. Glyphosate inhibits rust diseases in glyphosate-resistant wheat and soybean / P. Feng [еt al.] // PNAS. – 2005. – Vol. 102. – P. 17290–17295.
12. Устойчивость к глифосату трансгенного картофеля с геном аroA / Х. М. Пасалари [и др.] // Труды БГУ. – 2015. – Т. 10, ч. 1. – С. 123–126.
13. Tolerance and accumulation of shikimic acid in response to glyphosate applications in glyphosate resistant and non glyphosate resistant cotton (Gossypium hirsutum L.) / W. A. Pline [еt al.] / J. Agric. Food Chem. – 2002. – Vol. 50. – P. 506– 512.
14. Livak, K. J. Аnalysis of Relative Gene Expression Data using Real-Time Quantitative PCR and the 2–∆∆ct: Method Methods 25 / K.J. Livak // Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University. – P. 402–408.
Review
For citations:
Pasalari Ch., Tretiakova O.М., Еvtushenkov А.N. Induction of protective answer genes in potato leaves at bacterial infection and glyphosate treatment. Сrop Farming and Plant Growing. 2016;(3):37-39. (In Russ.)