cropЗемледелие и растениеводствоСrop Farming and Plant Growing2788-550Xcrop-648Research ArticleОВОЩЕВОДСТВОVEGETABLE GROWINGИдентификация сортообразцов чеснока озимого (Allium sativum L.) с использованием микросателлитных маркеровIdentification of winter garlic (Allium sativum L.) varieties using microsatellite markersКохтенковаИ. Г.KokhtenkovaI. G.

доктор с.- х. наук

СкоринаВ. В.SkorinaV. V.

доктор с.- х. наук

ДомблидесА. С.DomblidesA. S.

доктор с.-х. наук

Белорусская государственная сельскохозяйственная академияBelarusФедеральный научный центр овощеводстваRussian Federation202116052022034448Copyright © Кохтенкова И.Г., Скорина В.В., Домблидес А.С., 20222022Кохтенкова И.Г., Скорина В.В., Домблидес А.С.Kokhtenkova I.G., Skorina V.V., Domblides A.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://crop.belal.by/jour/article/view/648

В статье изложены результаты молекулярного анализа по идентификации сортообразцов чеснока озимого, отобранных из различных районов и областей Беларуси. Использованы 17 микросателлитных локусов для выявления генетического полиморфизма среди 54 селекционных образцов чеснока озимого (Allium sativum L.). В результате амплификации с микросателлитными локусами было получено 115 аллелей, с 6,7 аллели на локус в  среднем. По результатам проведенного исследования выявлено генетическое разнообразие между сортообразцами, которые были распределены по 10 кластерам. Выявлены различия по генотипу чеснока озимого как между сортообразцами, так и пунктами, в которых проводили отбор.

The article presents the results of molecular analysis for the identification of varieties of winter garlic, selected from different regions and regions of Belarus. We used 17 microsatellite loci to identify genetic polymorphism among 54 selection samples of winter garlic (Allium sativum L.). As a result of amplification with microsatellite loci, 115 alleles were obtained, with an average of 6,7 alleles per locus. Based on the results of the study, genetic diversity was revealed between the accessions, which were distributed over 10 clusters. Differences in the genotype of winter garlic were revealed both between the cultivars and the points at which the selection was carried out.

ReferencesКиселева, А. В. Хромосомная организация центромерных ретротранспозонов семейства TY3/GYPSY у Allium cepa L. и Allium fistulosum L. / А. В. Киселева, И. В. Киров, Л. И. Хрусталева // Генетика. – 2014. – Т. 50, № 6. – С. 670–676.Киселева, А. В. Хромосомная организация центромерных ретротранспозонов семейства TY3/GYPSY у Allium cepa L. и Allium fistulosum L. / А. В. Киселева, И. В. Киров, Л. И. Хрусталева // Генетика. – 2014. – Т. 50, № 6. – С. 670–676.Чесноков, Ю. В. Оценка меры информационного полиморфизма генетического разнообразия / Ю. В. Чесноков, А. М. Артемьева // Сельскохозяйственная биология. – 2015. – № 50 (5). – С. 571–578.Чесноков, Ю. В. Оценка меры информационного полиморфизма генетического разнообразия / Ю. В. Чесноков, А. М. Артемьева // Сельскохозяйственная биология. – 2015. – № 50 (5). – С. 571–578.Al-Zahim, M. Classification of genetic variation in garlic (Allium sativum L.) revealed by RAPD / M.Al-Zahim, H. J. Newbury, B. V. FordLloyd // Hort Science. – 1997. – Vol. 32 (6). – P. 1102–1104.Al-Zahim, M. Classification of genetic variation in garlic (Allium sativum L.) revealed by RAPD / M.Al-Zahim, H. J. Newbury, B. V. FordLloyd // Hort Science. – 1997. – Vol. 32 (6). – P. 1102–1104.Bozzini, A. Discovery of Italian fertile tetraploid line of garlic / A. Bozzini // Econ. Bot. – 1991. – № 45. – P. 436–438.Bozzini, A. Discovery of Italian fertile tetraploid line of garlic / A. Bozzini // Econ. Bot. – 1991. – № 45. – P. 436–438.Analysis of the genetic diversity of garlic (Allium sativum L.) by simple sequence repeat and inter simple sequence repeat analysis and agro-morphological traits / S. Chen [et аl.] // Biochem Syst Ecol. – 2014. – Vol. 55. – P. 260–267.Analysis of the genetic diversity of garlic (Allium sativum L.) by simple sequence repeat and inter simple sequence repeat analysis and agro-morphological traits / S. Chen [et аl.] // Biochem Syst Ecol. – 2014. – Vol. 55. – P. 260–267.Analysis of the genetic diversity of garlic (Allium sativum L.) germplasm by SRAP / S. Chen [et аl.] // Biochem Syst Ecol. – 2013. – Vol. 50. – P. 139–146.Analysis of the genetic diversity of garlic (Allium sativum L.) germplasm by SRAP / S. Chen [et аl.] // Biochem Syst Ecol. – 2013. – Vol. 50. – P. 139–146.New microsatellite markers for garlic, Allium sativum (Alliaceae) / C. P. Cunha [et аl.] // Am. J. Bot. – 2012. – Vol. 99 (1). – P. 17–19.New microsatellite markers for garlic, Allium sativum (Alliaceae) / C. P. Cunha [et аl.] // Am. J. Bot. – 2012. – Vol. 99 (1). – P. 17–19.Dice, L. R. Measures of the amount of ecologic association between species / L. R. Dice // Ecology. – 1945. – Vol. 26. – P. 297–302.Dice, L. R. Measures of the amount of ecologic association between species / L. R. Dice // Ecology. – 1945. – Vol. 26. – P. 297–302.A review of microsatellite markers and their applications in rice breeding programs to improve blast diseaseresistance / M. Gous [et аl.] // Int. J. Mol. Sci. – 2013. – Vol. 14. – P. 22499–22528.A review of microsatellite markers and their applications in rice breeding programs to improve blast diseaseresistance / M. Gous [et аl.] // Int. J. Mol. Sci. – 2013. – Vol. 14. – P. 22499–22528.Ipek, M. Rapid characterization of garlic clones with locus-specific DNA markers / M. Ipek, A. Ipek, P. W. Simon // Turk. J. Agric. For. – 2008. – Vol. 32. – P. 357–362.Ipek, M. Rapid characterization of garlic clones with locus-specific DNA markers / M. Ipek, A. Ipek, P. W. Simon // Turk. J. Agric. For. – 2008. – Vol. 32. – P. 357–362.Ipek, M. Molecular characterization of Kastamonu garlic: an economically important garlic clone in Turkey / M. Ipek, A. Ipek, P. W. Simon // Sci Hortic. – 2008. – Vol. 115 (2). – P. 203–208.Ipek, M. Molecular characterization of Kastamonu garlic: an economically important garlic clone in Turkey / M. Ipek, A. Ipek, P. W. Simon // Sci Hortic. – 2008. – Vol. 115 (2). – P. 203–208.Development and validation of new SSR markers from expressed regions in the garlic genome / M. Ipek [et аl.] // Scientia Agricola. – 2015. – Vol. 72 (1). – P. 41–46.Development and validation of new SSR markers from expressed regions in the garlic genome / M. Ipek [et аl.] // Scientia Agricola. – 2015. – Vol. 72 (1). – P. 41–46.Inter simple sequence repeat fingerprints for assess genetic diversity of Tunisian garlic populations / N. Jabbes [et аl.] // J. Agric. Sci. – 2011. – Vol. 3. – P. 77–85.Inter simple sequence repeat fingerprints for assess genetic diversity of Tunisian garlic populations / N. Jabbes [et аl.] // J. Agric. Sci. – 2011. – Vol. 3. – P. 77–85.Classification of genetic variation in garlic (Allium sativum L.) using SSR markers / M. Jo [et аl.] // Aust. J. Crop Sci. – 2012. – Vol. 6. – P. 625–631.Classification of genetic variation in garlic (Allium sativum L.) using SSR markers / M. Jo [et аl.] // Aust. J. Crop Sci. – 2012. – Vol. 6. – P. 625–631.Classification of genetic variation in garlic (Allium sativum L) using SSR markers / M. H. Jo [et аl.] // AJCS. – 2012. – Vol. 6 (4). – P. 625–631.Classification of genetic variation in garlic (Allium sativum L) using SSR markers / M. H. Jo [et аl.] // AJCS. – 2012. – Vol. 6 (4). – P. 625–631.Lampasona, S. G. Genetic diversity among selected Argentinean garlic clones (Allium sativum L.) using AFLP (Amplified Fragment Length Polymorphism) / S. G. Lampasona, L. Martínez, J. L. Burba // Euphytica. – 2003. – Vol. 132 (1). – P. 115–119.Lampasona, S. G. Genetic diversity among selected Argentinean garlic clones (Allium sativum L.) using AFLP (Amplified Fragment Length Polymorphism) / S. G. Lampasona, L. Martínez, J. L. Burba // Euphytica. – 2003. – Vol. 132 (1). – P. 115–119.Cross-amplification of SSR markers developed from Allium sativum to other Allium species / G. A. Lee [et аl.] // Sci Hortic. – 2011. – Vol. 128. – P. 401–407.Cross-amplification of SSR markers developed from Allium sativum to other Allium species / G. A. Lee [et аl.] // Sci Hortic. – 2011. – Vol. 128. – P. 401–407.Isolation and characteristics of eight novel polymorphic microsatellite loci from the genome of garlic (Allium sativum L.) / K. H. Ma [et al.] // Sci Hortic. – 2009. – Vol. 122. – P. 355–361.Isolation and characteristics of eight novel polymorphic microsatellite loci from the genome of garlic (Allium sativum L.) / K. H. Ma [et al.] // Sci Hortic. – 2009. – Vol. 122. – P. 355–361.Ohri, D. Phenology and genome size variation in Allium L. – a tight correlation / D. Ohri, K. Pistrick // Plant Biol. – 2001. – Vol. 3. – P. 654–660.Ohri, D. Phenology and genome size variation in Allium L. – a tight correlation / D. Ohri, K. Pistrick // Plant Biol. – 2001. – Vol. 3. – P. 654–660.Pearce, S. R. The Ty1-copia group retrotransposons of Allium cepa are distributed throughout the chromosomes but are enriched in the terminal heterochromatin / S. R. Pearce, U. Pich, G. Harrison // Chrom. Res. – 1996. – Vol. 4. – P. 357–364.Pearce, S. R. The Ty1-copia group retrotransposons of Allium cepa are distributed throughout the chromosomes but are enriched in the terminal heterochromatin / S. R. Pearce, U. Pich, G. Harrison // Chrom. Res. – 1996. – Vol. 4. – P. 357–364.Perrier, X. Data analysis methods / X. Perrier, A. Flori, F. Bonnot // Genetic diversity of cultivated tropical plants / eds.: P. Hamon, M. Seguin, X. Perrier, J. C. Glaszmann. – Enfield, 2003. – P. 43–76.Perrier, X. Data analysis methods / X. Perrier, A. Flori, F. Bonnot // Genetic diversity of cultivated tropical plants / eds.: P. Hamon, M. Seguin, X. Perrier, J. C. Glaszmann. – Enfield, 2003. – P. 43–76.Evolution of genome size across some cultivated Allium species / A. Ricroch [et al.] // Genome. – 2005. – Vol. 48. – P. 511–520.Evolution of genome size across some cultivated Allium species / A. Ricroch [et al.] // Genome. – 2005. – Vol. 48. – P. 511–520.Genetic structure and eco-geographical adaptation of garlic landraces (Allium sativum L.) in Iran / S. Shaaf [et al.] // Genet. Res. Crop. – 2014. – Vol. 61. – P. 1565–1580.Genetic structure and eco-geographical adaptation of garlic landraces (Allium sativum L.) in Iran / S. Shaaf [et al.] // Genet. Res. Crop. – 2014. – Vol. 61. – P. 1565–1580.BAC FISH analysis in Allium cepa / G. Suzuki [et al.] // Genes and Genetic Systems. – 2001. – Vol. 76. – P. 251–255.BAC FISH analysis in Allium cepa / G. Suzuki [et al.] // Genes and Genetic Systems. – 2001. – Vol. 76. – P. 251–255.Volk, G. M. Genetic diversity among US garlic clones as detected using AFLP methods / G. M. Volk, A. D. Henk, C. M. Richards // JASHS. – 2004. – Vol. 129 (4). – P. 559–569.Volk, G. M. Genetic diversity among US garlic clones as detected using AFLP methods / G. M. Volk, A. D. Henk, C. M. Richards // JASHS. – 2004. – Vol. 129 (4). – P. 559–569.Molecular genetic diversity and population structure of a selected core set in garlic and its relatives using novel SSR markers / W. G. Zhao [et al.] // Plant Breed. – 2011. – Vol. 130. – P. 46–54.Molecular genetic diversity and population structure of a selected core set in garlic and its relatives using novel SSR markers / W. G. Zhao [et al.] // Plant Breed. – 2011. – Vol. 130. – P. 46–54.

The authors declare that there are no conflicts of interest present.